WebJul 16, 2024 · The knocked-in iCre or CreERT2 were used for lineage tracing, which suggested nkx6.1 + cells are multipotent pancreatic progenitors that gradually restrict to bipotent duct; while id2a + cells are multipotent in both liver and pancreas and gradually restrict to ductal cells. WebGenomic DNA Preparation by Macherey-Nagel Tissue Spin Columns Using this kit according to the manufacturer’s protocol is the standard way for preparing genomic DNA at OTTC when it is intended for ddPCR (i.e. copy-number quantification). It will also work for conventional PCR genotyping.
The original and modified CRIS-PITCh systems (v1 and v2
WebMar 9, 2024 · The Cre/loxP system is a powerful tool for generating conditional gene knockout (KO) mice and elucidate gene function in vivo. CD19-Cre and Mb1-iCre … WebNov 1, 2024 · For the Sst locus, a P2A-iCre-T2A-mCherry cassette was inserted immediately upstream of the stop codon (C terminus fusion). For the Cck locus, iCre-P2A-mCherry-T2A cassette was inserted at the start codon (N terminus fusion). Knock-in mice were generated using the Easi- CRISPR method. bleach symbolism
Generation and characterization of CD19-iCre mice as a
WebpCAGGS-mTagBFP2-T2A-iCreERT2 (PV) Plasmid #128171 Purpose Monomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus) Depositor Mario Capecchi Insert mTagBFP2-T2A-iCreERT2 Use Cre/Lox Tags Expression Mammalian Mutation Promoter Availability Academic Institutions and Nonprofits only Enlarge pCAGGS-mTagBFP2-T2A-iCreERT2 … WebMar 30, 2024 · To generate the Lrig1T2A-iCreERT2 allele mice, G4 stem cell line from a C57BL/6Ncr x 129S6/SvEvTac F1 hybrid mouse embryo was targeted with a vector recombineered from a C57BL/6J BAC library clone (NGS sequence of the vector here). Mice were generated from two embryonic stem cell clones, 1c2 and 2f1 (see below for more … WebPB CAG-mTagBFP2-T2A-iCreERT2: CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon: Loading... 177309: PB CAG-sfGFP-T2A-iCreERT2: CAG-sfGFP-T2A-iCre-ERT2 piggyBac transposon: Loading... 178044: PB CAG-L-bpA-2xSV40pA-L-tdGFP-Pten_shRNAmir: CAG tdGFP Pten shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop) bleach synchronisation manette