Plvx-shrna2

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August undefined, 2024

WebJan 20, 2024 · 方法：根据GenBank提供的3β-HSD基因mRNA序列，设计合成3条干扰序列，退火后连接到PLVX-shRNA2-puro中，将重组慢病毒载体转染293FT细胞，收集病毒上 … Web中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电话0574-87917803

pLVX-shRNA2-Puro - lifescience-market.com

WebJan 20, 2024 · Methods: According to the mRNA sequence of 3β-HSD gene provided by GenBank, three interference sequences were designed and connected to PLVX-shRNA2-puro after annealing. The recombinant... WebAug 9, 2024 · Plasmids pLVX-shRNA2-m and PLVX-mcmv-ZsGreen1 were purchased from Biowit Technologies, Ltd. (China). pLVX-shRNA2-m was first digested with PstI and then filled in with Klenow. The larger fragment was then extracted and cleaved with BamHI. hoping for your immediate response

Optimized protocol for high‐titer lentivirus production and ...

WebpLVX-shRNA2 can be used as a plasmid expression vector and transfected into cells, or it can be packaged into viral particles and transduced into cells. Lentiviral particles derived … WebTakara Bio—Home hoping for your feedback

Down-regulation of microRNA-21 inhibits cell proliferation and invasion …

Addgene: Vector Database - pLVX-Puro

Web靶向ERRα基因的慢病毒重组载体及其构建方法和应用,深圳市南山区疾病预防控制中心,202410931991.3,发明授权,本发明公开了一种靶向ERRα基因的慢病毒重组载体，包括pLVX‑shRNA1表达载体的基本序列、抗性基因序列、多克隆位点序列和靶向ERRα基因的寡核苷酸链序列；所述寡核苷酸链序列包括正义链序列 ... WebThe pLVX-CXCR4-shRNA2 scramble was transfected 9062 X. HE ET AL. into 293 T cells at the logarithmic growth phase using Lipo 3000 (Thermo, L3000008). MSCs were inoculated at a density of 1*10 per well [14], added with 2 ml F12K medium, and culture at 37°C and 5% CO 2 in saturated humidity. hoping for your immediate actionWebpLVX-shRNA2 is an HIV-1-based, lentiviral expression vector designed to express a small hairpin RNA (shRNA) for RNA interference (RNAi) studies. Expression of your shRNA is … long term rental properties in goolwa sa

"WebDec 3, 2024 · The pLVX-shRNA2 and pLVX-IRES-ZsGreen1 plasmids (Clontech, USA) were selected as the lentiviral vectors for PC4 knockdown and overexpression, respectively. The target sequences for PC4 knockdown were PC4 sh1 GAAGGAACAGATTTCTGACAT; PC4 sh2 GACAGGTGAGACTTCGAGA; and PC4 sh3 GCAAAGTGCTAATTGATAT. " - Plvx-shrna2

Plvx-shrna2

Optimized protocol for high-titer lentivirus production and ...

WebMar 8, 2024 · The pLVX-shRNA2 lentiviral transfer plasmid was associated with the highest lentiviral titer, while both pLVX-shRNA2 and psi-LVRU6GP plasmids were associated with efficient RNA interference in target cells. WebSep 4, 2024 · The constructed plasmid was named pLVX-ShRNA2 -Puro-Homo-ATG5-853, and it was packaged and transferred into HEK293 cells. Transfection efficiency was observed at 24 and 72 h after transfection, and the lentivirus pLVX-ATG5 titer was measured as 8 × 10 7 TU/mL. MDA-MB-231 cells were infected, and infection efficiency was …

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WebMar 21, 2024 · PLXNA2 (Plexin A2) is a Protein Coding gene. Diseases associated with PLXNA2 include Tetralogy Of Fallot and Schizophrenia . Among its related pathways are … WebAug 14, 2015 · zt123 pLVX-shRNA1 载体 Clontech RNAi 慢病毒载体系统. zt124 pLVX-shRNA2 载体 Clontech RNAi 慢病毒载体系统. zt125 pSIH1-H1-CoGFP 载体 SBI RNAi 慢病毒载体系统. zt126 pGIPZ empty 载体 RNAi 慢病毒载体系统. zt127 pGIPZ control 载体 RNAi 慢 …

WebpLVX-shRNA2 is an HIV-1-based, lentiviral expression vector designed to express a small hairpin RNA (shRNA) for RNA interference (RNAi) studies. Expression of your shRNA is driven by the RNA Pol III-dependent, human U6 promoter (PU6), located just upstream of the MCS. pLVX-shRNA2 can be used as a plasmid expression vector and transfected into ... WebOct 11, 2024 · The original CMV IE promoter of pLVX-shRNA2 was replaced with a mouse PGK promoter (PCR and cloned by primers 5’–ACACGAATTCTACCGGGTAGGGGAGGCGCT and 5’–ACACCCCGGGCGAAAGGCCCGGAGATGAGG) to generate the pLVX2-shRNA2-PGK vector which drives persistent ZsGreen (enhanced GFP) expression. Oligos carrying short …

WebJul 19, 2012 · After transcription by RNA polymerase II (RNA Pol II), the first step in the biogenesis of miRNA involves the cleavage of the primary miRNA transcript (pri-miRNA) by Drosha in the nucleus to produce a ∼60–100 nt hairpin-like structure called the precursor miRNA (pre-miRNA). WebMar 8, 2024 · The pLVX-shRNA2 lentiviral transfer plasmid was associated with the highest lentiviral titer, while both pLVX-shRNA2 and psi-LVRU6GP plasmids were associated with …

WebFeb 1, 2024 · The targets of LACTB shRNA sequences were 5′-CCATGGTTGCTCTTGCCAA-3′ and 5′-GCCATAGTAGAGAGAGCTT-3’. Oligos containing the scrambled or non-targeted …

WebpLVX-shRNA1 Vector Information. Clontech Laboratories, Inc. www.clontech.com Protocol No. PT4051-5 2 Version No. PR073571. Use. pLVX-shRNA1 is available in the Lenti-X™ shRNA Expression System (Cat. No. 632177). Before it can be transduced into target cells, the vector must be cotransfected into 293T cells with our Lenti-X HTX Packaging hoping for your immediate attentionWebJan 30, 2024 · Suppression of IAPP expression by pLVX-ShRNA2-Puro-IAPP exerted opposite effects of increasing pro-apoptotic Bax and caspase-3 gene expression (Figures 5a–c). Upregulation of IAPP also increased ... long term rental properties in gibraltarWebAug 13, 2024 · Argonaute2 (Ago2) is a key component of the RISC that can directly degrade mRNA by a slicing mechanism 21, 22, we performed RIP assays using the AGO2 antibody to further confirm the interaction... long term rental properties spainWebpLVX-shRNA2 lentiviral vector (Clontech. Laboratories Inc., Palo Alto, CA, USA) was used to construct the pLVX-anti-miR-21 RNAi. Meanwhile, the oligonucleotides for the miR-21 were constructed and synthesized by the Shaihai Generay Biotech. Co. Ltd. (Shanghai, China). long term rental properties in nerja spainWebFast accurate construct design for all major molecular cloning techniques. Validate sequenced constructs using powerful alignment tools. Customize plasmid maps with … long term rental pcb flWebAfter annealing in vitro, insert pLVX-shRNA-puro construct recombinant plasmid, then enzyme digestion and sequencing analysis. The lentiviral-shRNAs were transient-transfected into K1 cells. TFF3 mRNA and protein levels were test by real-time PCR and western blot respectively in K1 cells at 48h post transient-transfected. long term rental properties in southern spainWebpLVX-shRNA2-Puro Description Plasmid type:RNAi vectorPromoter:U6Size:8998 bpReporter gene:ZsGreen1Resistance (s):Ampicillin (Amp)Selectable markers:Puromycin Caution: 1. … long term rental properties in mojacar