Phospho irf3

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August undefined, 2024

WebSep 19, 2013 · IRF3 is a constitutively expressed transcription factor that resides in the cytoplasm and dimerizes and translocates to the nucleus upon phosphorylation ( 6 ). … WebNational Center for Biotechnology Information

STING-IRF3 pathway links endoplasmic reticulum stress …

WebPrimary Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859) Protein Recombinant Human IRF3 protein (ab205216) Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab205718) View more associated products Overview Product name Anti-IRF3 (phospho S396) antibody See all IRF3 primary antibodies Description WebPhospho-IRF3 (S386) ELISA Kit (ab279833) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated IRF3 protein in your experimental model system, you can verify pathway activation in your cell lysates. sidestory是什么意思

Irf3 Cell Signaling Technology Inc Bioz

IRF3 is a member of the interferon regulatory transcription factor (IRF) family. IRF3 was originally discovered as a homolog of IRF1 and IRF2. IRF3 has been further characterized and shown to contain several functional domains including a nuclear export signal, a DNA-binding domain, a C-terminal IRF association domain and several regulatory phosphorylation sites. IRF3 is found … WebApr 13, 2024 · Antibodies. The following antibodies were used: mouse monoclonal antibodies to α-tubulin (Santa Cruz, sc23948), GAPDH (Santa Cruz, sc32233), phospho-H2A.X (Ser139) (EMD Millipore, 05-636), anti ... WebPhospho-IRF-3 (Ser396) Antibody detects endogenous levels of IRF-3 only when phosphorylated at Ser396. This antibody does not cross-react with other family members … Monoclonal Antibody for studying IRF-3 (Ser396) phosphate. Cited in 635 … side story taion

Phospho-IRF3 (Ser386) cellular HTRF kit Cisbio

Phospho-IRF-3 (Ser396) Antibody Cell Signaling Technology

WebJul 7, 2024 · Phospho-S365 acts as a docking site for IRF3, a transcription factor that is phosphorylated and activated by TBK1, leading to transcriptional induction of type I interferons (IFNs). WebSep 19, 2013 · IRF3 is a constitutively expressed transcription factor that resides in the cytoplasm and dimerizes and translocates to the nucleus upon phosphorylation ( 6 ). Phosphorylated IRF3 induces IFN-β during viral infection, but may also contribute to inflammatory cytokine response to LPS ( 7 ). the pledge to texasWebAll lanes : Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493) at 0.2 µg/ml (purified) Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates Lane 2 : … the pledge to the american flag

"WebIRF3 Antibodies Antibodies that detect IRF3 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry and Peptide array. These antibodies target IRF3 in Human, Mouse, Rat, Non-human primate and Porcine samples. " - Phospho irf3

Phospho irf3

WebPhospho-IRF3 (Ser396) Antibody (720012) in ICC/IF. Immunofluorescence was performed on fixed and permeabilized HeLa cells treated with Calyculin A (50 ng/mL/20 min) for … WebAnti Phospho Irf3 Ser396, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

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WebDetect and quantitate cellular phospho-IRF3 (Ser386) using a homogeneous AlphaLISA® SureFire® Ultra™ assay. We are now two standalone organizations Life Sciences & … WebJun 10, 2016 · Each plate also contained positive controls (p65, MAVS, IRF3-5D (constitutively active IRF3), IKK-SE (constitutively active IKK) TRIF),negative controls (pcDNA3.1 vector, GFP, pCherryvectors), emptywells. HEK293T/NF-kB-luciferasecell line high-throughputtrans- fection Fugene6transfection reagent (Roche).

WebIRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. …

WebWestern Blot using Phospho-IRF3 (Ser386) Polyclonal Antibody ( Product # PA5-78025 ). Untreated (–) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Phospho-IRF3 (Ser386) Polyclonal Antibody ( Product # PA5-78025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody ... WebIRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine/threonine cluster.

WebMar 30, 2024 · Similarly, the IRF7, IRF1, and phospho-IRF3 levels hiked after two days of SARS-CoV-2 infection. These findings suggested that IRF7, IRF1, and IRF3 were stimulated following HCoV infections.

WebJun 1, 2024 · IRF-3 Antibody (SL-12) is a mouse monoclonal IgG 1 κ IRF-3 antibody, cited in 73 publications, provided at 200 µg/ml raised against recombinant IRF-3 fusion protein corresponding to human IRF-3 (amino acids 56-427) IRF-3 Antibody (SL-12) is recommended for detection of IRF-3 of mouse, rat and human origin by WB, IP, IF and IHC … the pledge to the christian flagWebPhospho-IRF3 (S386) ELISA Kit (ab279833) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human … the pledge to the holy bibleWebSep 1, 2024 · To systematically search for the phosphorylation sites on IRF3 in vivo, we carried out tandem affinity purification of recombinant double-tagged IRF3 expressed in HEK293 cells, coupling with mass spectrometric analysis (Fig. 1 A and B). Several phospho-peptides were identified with different color highlighted in the whole amino acid sequence … the pledge trailerWeb"Phospho-IRF3 (Ser396) antibodies" comparison At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. the pledging of receivables quizletWebApr 23, 2024 · We focused on the phosphorylation of IRF3, which is a downstream factor of the cGAS pathway . As shown in Figure 2A, the expression of phosphorylated-IRF3 (p-IRF3) was increased in HNSCC cells after X-ray irradiation, and cGAS knockdown suppressed the radiation-increased p-IRF3. These results suggest that ionizing radiation activates IRF3 ... sides to serve with chicken pot pieWebIntracellular Delivery of Lipopolysaccharide Induces Effective Th1-Immune Responses Independent of IL-12 Sachiko Watanabe1., Joe Inoue1,2*. 1Department of Biosciences, School of Science and Graduate School of Science, Kitasato University, Japan, 2Department of Immunology and Cell Biology, Graduate the pledge traduction françaisWebPhosphorylated IRF3 forms a dimer that translocates to the nucleus and induces type I IFNs and other cytokines. After activation by STING, TBK1 and its homolog IκB kinase epsilon (IKKε) can both lead to activation of the IKK complex, which then activates the transcription factor nuclear factor κB (NF-κB) (11, 12). the plees to be