WebbClose You have no items in your shopping cart.. Home; Plasmid; p53- His2 Model: PVT11251 Webb+ 5 µg pHis2.1 20 µl Note: To denature carrier DNA, heat to 95–100°C for 5 min, then cool rapidly in an ice bath. NOTE NOTE Protocol No. PT3529-1 Version No. PR7Z2445 32 3. Add competent cells and gently mix. 4. Add PEG/LiAc and gently mix. 5. Incubate at 30°C . NOTE: Mix cells every 10–15 min. www.clontech.com 50 µl 500 µl 30 min 600 µl 2.5 ml
Setaria italica SiWRKY89 enhances drought tolerance in …
Webb26 mars 2024 · The promoter sequence (1800 bp) of MdMATE8 (MDP0000175055) was inserted into the pHIS2.1 vector (Clontech, Palo Alto, CA, United States), which contained … Webb1 mars 2024 · MdGSTF6-p-pHIS2 vectors were first grown on −Trp/−His medium containing 3-amino-1,2,4-triazole (3-AT) for screening. The concentration of 3-AT used was 100 mM. dicks sporting good in reno
Y187-pHis2YeastOne-HybridMediaKit 使用说明书
WebbBait Vector Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > bait vector phis2. bait vector phis2 TaKaRa is a verified supplier TaKaRa ... Webb1 mars 2024 · C. Yeast one-hybrid assay detect the specific binding ability of VvWRKY30 to W-BOX. pGADT7-empty and pHIS2.1-W-BOX, AD- VvWRKY30 and pHIS2.1-empty were … WebbProbe 2 and Probe 4 sequences as wells mutant Probe 2M and Probe 4M sequences were cloned to pHIS2.1, which drives the expression of HIS3 . GBFs were cloned to pGADT7. Yeast cells were co-transformed with a combination of the indicated plasmids or empty vector and plated onto nonselective (−Leu/−Trp) and selective ... dicks sporting good internships