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Phinnygene aol.com
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WebbPlasmid pAS_4xMx PylT_GFP 150TAG from Dr. Simon Elsaesser's lab contains the insert sfGFP and is published in ACS Chem Biol. 2024 Sep 27. doi: 10.1021/acschembio.8b00571. This plasmid is available through Addgene. Webb1) problem: "optGene didn't find any set". possible reason: probably, the limit of time or the number of generations has not been enough. Another explination is that the solver is not suited for solving optGene. solution: Try with a higher number for inputs "TimeLimit" of "Generations" or using another solver.
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